DNA

Part:BBa_K5319678:Design

Designed by: Wang Xuanhao   Group: iGEM24_ZJU-China   (2024-10-01)


λ peptide-GS linker-vioD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 309
    Illegal PstI site found at 892
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 465
    Illegal SpeI site found at 309
    Illegal PstI site found at 892
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 309
    Illegal PstI site found at 892
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 309
    Illegal PstI site found at 892
    Illegal NgoMIV site found at 987
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

λ peptide-GS linker binds proteins with this junction together through a protein skeleton


Source

vioD is from NCBI library.

References